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Jay D. Dias
Naresh T. Deoli
Deborah Rogers
Armin de Vera
Karen M. Smith
Robin Schmeider-Broussard
Harry J. Whitlow

The development of a facility for irradiation of murice and primate tissue presents several challenges. First, extremely low fluences (10⁶-10¹¹ ions cm⁻²) are required to deliver doses of 1 mGy - 50 Gy using MeV proton. Second,the tissues, particularly from non-human primate, may be infectious to humans. Third, protection of irradiated tissue samples from contamination by mold and bacteria is critical. Here we present an ultra-low flux irradiation facility and associated fluence control for biological cell and tissue irradiation under sterile biosafety level 2 (BSL-2) conditions suitable for use with low energy (1-3.4 MeV) protons. The operation is demonstrated using murine astocytes.

Submitted 9 February 2019, Received in revised form 28 February 2019, Accepted 4 March 2019

https://doi.org/10.1016/j.nimb.2019.03.002

Ultra low-flux irradiation
MeV ion microprobe
Tissue irradiation
Biosafety
Murine astrocytes